[Plant-education] Re: Growing Rhizobium in a lab

schouse at gmail.com schouse at gmail.com
Fri Jan 6 03:01:51 EST 2006


Thanks,

So in effect, it can be grown in lab. Could you point me to resources
where the optimum growth parameters are mentioned or something like
that?

I'm an masters in technology student in a college in India and am
looking to do a project in a bioprocess control system design to
culture this bacteria.

Thanks,
Mohit.

Jon Monroe wrote:
> Hi Mohit,
>
> I used to work on legume root nodules so I'll try to answer your
> question.  Rhizobia have two major lifestyles, free living in soil (or a
> culture flask), and as an intracellular symbiont in root nodules of
> legumes.  In the free living state they have cell walls and are easy to
> grow.  During root nodule development Rhizobia differentiate in various
> ways - they become larger and sometimes branched, express a number of
> different genes associated with N2 fixation, and apparently lose the
> ability to dedifferentiate and reproduce! (I didn't know that when I
> started this response).  Some strains do grow slowly (e.g.
> Bradyrhizobium, also referred to as the "slow growers"), but they do
> grow in culture.
>
> Hope this helps.
>
> Jon
>
>
> schouse at gmail.com wrote:
> > Hi,
> >
> > Is it possible to culture rhizobium bacteria in a lab bioreactor? I've
> > read somewhere that these bacteria lack a cell wall and cannot be
> > easily cultured?
> >
> > Regards,
> > Mohit.
> >
> > _______________________________________________
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> > Plant-ed at net.bio.net
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>
>
> --
> ---------------------------------
>   Jonathan Monroe, Professor
>   Department of Biology, MSC 7801
>   James Madison University
>   Harrisonburg, VA 22801
>   office: 304 Burruss Hall
>   phone: 540-568-6649
>   fax: 540-568-3333
>   e-mail: monroejd at jmu.edu
>   http://www.jmu.edu/biology/
> ---------------------------------



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