[Plant-education] stain vascular tissue?
(by Doug.Jensen At Converse.edu)
Wed Feb 7 17:55:57 EST 2007
Toluidine blue also works for lignified and suberized tissues, and it's
safer than phloroglucinol. I have never mixed it up, so I don't know
the recipe, but I've seen references to 0.1% and 0.05%.
I still prefer phloroglucinol myself, but that may be because I like the
Douglas P. Jensen
Chair of Biology
Spartanburg, South Carolina, 29302
douglas.jensen At converse.edu
From: plant-ed-bounces At oat.bio.indiana.edu
[mailto:plant-ed-bounces At oat.bio.indiana.edu] On Behalf Of David W.
Sent: Wednesday, February 07, 2007 3:17 PM
To: plant-ed At magpie.bio.indiana.edu; sjohnson
Subject: Re: [Plant-education] stain vascular tissue?
Phloroglucinol can be used to stain lignified cells (xylem). Make up
EtOH, 95% 100 ml
HCl (conc) 16 ml
Phloroglucinol 0.1 g
Place a drop of phloroglucinol solution onto the tissue (thin
section) and incubate 30-60 min. [temperature is not indicated!]
Result: Lignified cell walls stain red. Because the dye fades in a
short time, the preparation is not permanent.
This is taken directly from page 149 of Ruzin, Steven E. 1999.
Plant Microtechnique and Microscopy. New York: Oxford University
>can anybody suggest a stain for xylem &/or phloem of 'live' plant
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David W. Kramer, Ph.D.
Asst. Prof. of Evolution, Ecology, and Organismal Biology
Ohio State University at Mansfield
1680 University Drive
Mansfield, OH 44906-1547
Phone: (419) 755-4344 FAX: (419) 755-4367
e-mail: kramer.8 At osu.edu
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