[Plant-education] Re: stomata - dental impression material

tj via plant-ed%40net.bio.net (by t-jacobs from uiuc.edu)
Fri Mar 2 10:16:31 EST 2007


In article <mailman.128.1172590778.5139.plant-ed from net.bio.net>,
 Carl Pike <carl.pike from fandm.edu> wrote:

> Has anyone used dental impression material for stomatal counting?  A 
> material I used when overseas (Xantopren VL Plus) is not available in 
> the US.  Do you know of a comparable material sold here?  Thanks.

The current choice is dental impression material, "light body, surface 
activated ISO 4823, Type 3, low consistency Polyvinylsolixane 
(addition-type)"  President Plus Jet (Art.#4600) from Coltene Whaledent 
(Cuyahoga Falls, OH, productinfo from coltenewhaledent.com).  Comes in 48 ml 
doublebarreled syringe (2/box) that requires their dedicated injector 
gun (DMA-50).   Works nicely (Arabidopsis) for going back to same leaf 
domain for developmental series.  Not so well for surveying hundreds of 
leaves (once each) for total *counts* for index and density 
determinations.  For that, we use Revlon  960 Double Twist nail varnish 
and double stick tape on a slide.

I've heard from a lab downstairs about some superfast hardening nail 
varnish from Austria that works exceedingly well.  But no experience 
with that.

Tom Jacobs
UIUC



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