[Plant-education] extraction of mtDNA

[Higbie, Sarah]

Hello All,

I am wondering if anyone has any tips for isolating quality
mitochondrial DNA from leaves. We have tried a few protocols (Triboush
et al. 1998. Plant Mol Biol Rep 16: 183-189; Dellaporta et al. 1983.
Plant Mol Biol rep 1:19-21; Scotti et al. 2001. Plant Mol Biol Rep
19:67a-67h) with limited success. We will be using the mtDNA with PCR. 

 

Any help is greatly appreciated. 

 

Sarah Higbie, Ph.D.

Department of Biology

Saint Joseph College

1678 Asylum Avenue

West Hartford, CT  06117-2791

Office: (860) 231-5768

FAX: (860) 231-8396

E-mail: shigbie from sjc.edu