[Plant-education] extraction of mtDNA
(by shigbie from sjc.edu)
Tue Feb 26 09:23:43 EST 2008
I am wondering if anyone has any tips for isolating quality
mitochondrial DNA from leaves. We have tried a few protocols (Triboush
et al. 1998. Plant Mol Biol Rep 16: 183-189; Dellaporta et al. 1983.
Plant Mol Biol rep 1:19-21; Scotti et al. 2001. Plant Mol Biol Rep
19:67a-67h) with limited success. We will be using the mtDNA with PCR.
Any help is greatly appreciated.
Sarah Higbie, Ph.D.
Department of Biology
Saint Joseph College
1678 Asylum Avenue
West Hartford, CT 06117-2791
Office: (860) 231-5768
FAX: (860) 231-8396
E-mail: shigbie from sjc.edu
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