[Plant-education] KCN effect on DCPIP reduction of chloroplasts
(by KOdam from woodbridge.suffolk.sch.uk)
Tue Jan 11 04:52:18 EST 2011
Dear Douglas Bielenberg (or whoever receives this message as the e-mail link didn't go directly to Douglas Bielenberg),
I found your article http://www.bio.net/mm/plant-ed/2005-February/007802.html while looking for an experiment on spinach and DCPIP.
I was impressed by the comment that it was a 'robust lab' as the instructions I have been given don't work at all. My DCPIP when made to the required concentration is almost colourless to start with and the chloroplast mix is a murky green which would mask any colour change in the DCPIP. I assume the content of the mixtures have to be sufficiently precise to ensure that a colour change is seen within a reasonable time.
I would very much appreciate a copy of the instructions you used (if you still have them 5 years later!) so that our students have a chance of seeing the experiment working properly.
Dr K D Odam
More information about the Plant-ed