R: RNA from seeds, some more problems

ES at UK.AC.AFRC.JII ES at UK.AC.AFRC.JII
Mon Aug 31 11:16:00 EST 1992


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Subj:	RNA from siliques

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Brian,
I also got a rusty color contaminant in silique
RNA preps with the phenol/LiCl method.  I switched
to guanidinium thyocyanate/ CsCl and got clean RNA.
Some references for this method are:
Chirgwin et al Biochemistry 18:5294-99 (1979)
Kopczynski and Scandalios MGG 203:185-8 (1986)
and Current Protocols in Molecular Biology 4.2
Hope this helps.
P.S. The rusty contaminant hung in the wells of
agarose gels and the RNA still ran fine.
Linda Castle, Botany, Oklahoma State University



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