opines, Agrobacterium tumifaciens, and heterophyllic aquatic plants

[Scott Keefer]

	I am currently doing a research project in which I introduce  
Agrobacterium tumefacicens into a heterophyllic aquatic plant.  In order  
to confirm that a DNA transfer has occurred, I must measure if opine  
synthesis is present.  If anyone has any methods in how to measure this  
compound, our can suggest any literature about opines, it would be greatly  
appreciated.

Thank You.
Scott Keefer
Allegheny College
11/19/92