Tissue culture of bambooK

baumlj at cgsvax.claremont.edu baumlj at cgsvax.claremont.edu
Tue Oct 27 22:36:30 EST 1992



In article <9210222347.AA19730 at net.bio.net>, MMUNOZ at UCRVM2.BITNET ("Miguel E. Mu:oz Fonseca") writes:
> I'll greatly appreciate to receive information about propagation of bamboo by t
> issue culture methods, specially thick-walled bamboos.  As part of my thesis wo
> rk I've tried to propagate a kind of giant bamboo Dendrocalamus giganteus, howe
> ver in most of the paper I've read they have work with seeds or zigotic embryos
> , this kind of explant easly forms embryogenic callus, then somatic embrios and
>  afterthat regenerate whole plants.  However the specie I'm working with flower
> s once every 150-180 years and after the plant dies, so it's impossible for me
> to get flowers or seeds.  I've tried with vegetative tissue and some of them de
> velop callus that are neither embryogenic nor organogenic, and I've a serious p
> roblem, all the callus becomes necrotic and oxidized very easily and soon.  Hav
> e anybody work with bamboo tissue culture, or have deal with this kind of probl
> em in other Poaceae or graminae species?.  I'll really appreciate your advice.
> Thanking in advance,
>                              Miguel Munoz Fonseca  MMUNOZ AT UCRVM2
>                              Centro de Investigacion en Biologia Celular y
>                              Molecular.  Universidad de Costa Rica.


You might want to contact the president of the American Bamboo Society to
find out if he knows if anyone has had success in such tissue culture:
Gerald Bol, President, ABS, 666 Wagnon Rd., Sebastapol, CA 95472.



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