fixation protocols for electron microscopy of plant tissues (fwd)

Rod Bonfiglioli rbonfigl at waite.adelaide.edu.au
Sun Sep 13 15:28:31 EST 1992


Forwarded message:
> From BIOSCI-REQUEST at genbank.bio.net Sun Sep 13 14:05:20 1992
> From: rbonfigl at waite.adelaide.edu.au (Rod Bonfiglioli)
> Message-Id: <9209130431.AA25970 at schooner.waite.adelaide.edu.au>
> Subject: fixation protocols for electron microscopy of plant tissues
> To: plant-biology at genbank.bio.net
> Date: Sun, 13 Sep 92 14:01:41 CST
> X-Mailer: ELM [version 2.3 PL11]
> 
> >
> > Your article/letter follows:
> > Path: schooner!rbonfigl
> > Date: 13 Sep 92 04:14:10 GMT
> > Message-ID: <rbonfigl.716357650 at schooner>
> > Newsgroups: bionet.plants
> > Distribution: world
> > Subject: electron microscopy
> > Summary: help needed in T.E.M. fixation protocols.
> > Keywords: fixation, protoplasts,cellwalls
> > Organization: The University of Adelaide, Waite Campus
> > Faculty: Agricultural & Natural Resource Sciences
> > I am having some trouble getting adequate fixation of plant tissues in the
> > expected length of time, ie; that recommended in most protocols.  I have tried
> > infusion under mild vacuum, and i am now thinking of enzymatically digesting
> > away the plant cell walls and other impermeable tissues before i fix and embed.
> > Is there anyone else out there in this group who uses electron microscopy
> > on a regular basis?  i could use some advice here.
> >
> > thanx in advance
> > rod bonfiglioli
> > waite institute, university of adelaide, south australia.
> >
> > email rbonfigl at schooner.waite.adelaide.edu.au
> >
> 
> 




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