MSc/Phd studentship in plant/fungal interactions

frist at ccu.umanitoba.ca frist at ccu.umanitoba.ca
Wed Apr 28 16:19:00 EST 1993


                        GRADUATE RESEARCH ASSISTANTSHIP

               MOLECULAR BIOLOGY OF PLANT/PATHOGEN INTERACTIONS

PROJECT ABSTRACT - We shall apply transgenic plant technology to
investigate disease resistance mechanisms in canola (Brassica napus),
and to test the efficacy of the constitutive expression of a Brassica
defense gene against important canola diseases, in particular Blackleg
(Leptosphaeria maculans). Specifically our objectives are:
   1. To obtain genomic clones for Brassica homologues of the pea
      disease resistance response gene drr49.
   2. To transform Brassica with a chimeric 35S-drr49 Brassica gene and
      to determine whether constitutive expression of this gene
      enhances or otherwise affects resistance to Brassica pathogens.
   3. To investigate the expression of the Brassica drr49 gene in
      transgenic plants containing Brassica drr49 promoters fused with
      a reporter gene. The expression of these reporter genes in
      response to pathogens will make it possible to pinpoint the cells
      that express these genes, and to observe the timing of gene
      induction. Transgenic plants will be challenged with both
      incompatible and compatible fungi in order to detect differences
      in gene expression between resistant and susceptible
      interactions, respectively.  
This project complements work funded by the Western Grains Research
Foundation, in which Pea drr49 genes are being transformed into
Brassica. Together, these projects have the potential to generate
Brassica plants resistant to Blackleg or other canola diseases. At the
same time, insights gained into the mechanisms of disease resistance
may help guide future efforts in the genetic engineering of disease
resistance. 

QUALIFICATIONS - Qualified applicants must have a Bachelor's or
Master's degree in Genetics, Biochemistry, Plant Pathology, or related
field. Experience with PCR, DNA cloning and sequencing and cloning, and
plant transformation is also desireable.  Although candidates with a
strong background in recombinant DNA techniques are preferred, those
with strengths in other areas of biochemistry or in plant pathology 
will also be considered.
 
THE POSITION - Funding to cover tuition and stipend for an MSc. or PhD.
student is available immediately through March 1996. The search will
continue until a suitable applicant is found.  

THE UNIVERSITY, WINNIPEG AND MANITOBA - The University of Manitoba is
one of Canada's major centers for agricultural research. Other academic
and research institutions in Winnipeg include the Agriculture Canada
Station, the U. of M. Health Sciences campus and the University of
Winnipeg. With a cosmopolitan population of over 600,000, Winnipeg
offers a rich mixture of culture and entertainment, including the Royal
Winnipeg Ballet, and the Winnipeg Symphony Orchestra, as well as the
NHL Jets and CFL Blue Bombers. The comparatively low cost of living,
clean air and friendly people combine to make Winnipeg a very liveable
city. Winnipeg is at the interface of the prairie and boreal forest,
and the Manitoba lake country offers great opportunities for boating,
fishing and wilderness camping. 

APPLICATIONS - Send a curriculum vitae including a brief (one page)
description of either your research experience or research interests.
Promising applicants will be asked to submit a formal application to
the Faculty of Graduate Studies.  

           Dr. Brian Fristensky
           Department of Plant Science
           University of Manitoba
           Winnipeg, MB R3T 2N2  CANADA
  
Phone: 204-474-6085   FAX: 204-261-5732   Email: frist at ccu.umanitoba.ca

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