Martin Hughes Biochem
mjgh at mbfs.bio.cam.ac.uk
Thu Aug 5 10:11:27 EST 1993
In article <1993Aug5.144026.4471 at gserv1.dl.ac.uk> kanyuka at afrc.ac.uk writes:
>I make potato cDNA lib in lambda-gt11 and I have to check how
>is it. I'll screen it with RUBISCO DNA probe.
Sorry that this is not a direct answer, but just a point to watch-
Make sure that your RuBisCo probe is for rbcS and not rbcL. The
large sub-unit gene is coded for by the chloroplast, and chloroplast
mRNA does not have a poly(A) tail. Since most cDNA libraries are
constructed using oligo (T) as a primer, the rbcL message will not
be represented in a cDNA library.
Martin J. G. Hughes
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Email: mjgh at mbfs.bio.cam.ac.uk OR hughes at rkna50.riken.go.jp
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