SDS, detergents, Agro and cell permeability?

Ian Murray murrayian at PHIBRED.COM
Fri Aug 13 16:05:18 EST 1993


Dear netters,

I want to increase the permeability of plant cell membranes, to increase the
efficiency of Agrobact mediated transformation.  What conc. of detergent, SDS
or other, should I use to increase the permeability (and wetting with the 
bacteria in solution) but not kill or lyse the cells?

I was thinking of 0.001 to 0.0001% SDS.  ANY REFERENCES or INFO will be greatky
appreciated.

Murrayian at phibred.com

Ian Murray
*smile*



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