SDS, detergents, Agro and cell permeability?
murrayian at PHIBRED.COM
Fri Aug 13 16:05:18 EST 1993
I want to increase the permeability of plant cell membranes, to increase the
efficiency of Agrobact mediated transformation. What conc. of detergent, SDS
or other, should I use to increase the permeability (and wetting with the
bacteria in solution) but not kill or lyse the cells?
I was thinking of 0.001 to 0.0001% SDS. ANY REFERENCES or INFO will be greatky
Murrayian at phibred.com
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