staining phloem tissue
brunner at informatik.uni-muenchen.de
Thu Dec 9 08:56:54 EST 1993
I know one nice staining method that we used in our botany basic course:
Put the tissue into a drop of safranine solution and add one drop of
astrablue solution. After a few minutes (3..5) remove the staining
solutions with a paper handkerchief and put some drops of 90% ethanole
onto the tissue, and remove the coloured ethanole also. After that
you can repeat this step with ethanole, if still there is too much
colour around to get a clear sight.
The xylem parts are bright blue, the phloem is red coloured (I hope
that I didn't change this...).
This is a very easy and nice method to differentiate xylem and phloem.
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