re Hygromycin B, from Lenny B.
J Preiss--Seq Anal
preissj at CLVAX1.CL.MSU.EDU
Wed Feb 10 23:09:00 EST 1993
I don't know the cheapest source of Hygromycin, but I can tell you
how to cut your Hyg costs by 10 fold. Just drop your concentration down
10 fold. I tested the growth of Agro (LBA4301) on 523 medium for 1 week
and found that 1000 fold below published levels (20ug/ml) still completely
inhibits growth. I then switched to 2ug/ml Hyg for my plant transformations
and have had no problem with Agro growth on 3 and 4 week old plates (which
my student forgot to transfer). Good Luck.
Dr. Leonard N. Bloksberg
PreissJ at clvax1.cl.msu.edu
Dept. of Biochemistry
Michigan State University
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