Protoplasts and Cell Walls

Martin Hughes Biochem mjgh at
Thu Jul 29 05:38:14 EST 1993

Hello Everyone!

Does anyone have any idea as to how long after "making" them, 
protoplasts begin to regenerate their cell wall?  
How much longer does it then take for the cell wall to be 
fully formed?
Also, again with protoplasts, does anyone know of work/data
on the physical and biochemical state of protoplasts following
isolation? - How "ill" are they following enzymatic digestion
of their cell wall + dumping into sorbitol?
All suggestions/guesses welcome!
Martin J. G. Hughes
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Email: mjgh at  OR  hughes at

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