thaumatin

LERICKSO at CROP.UOGUELPH.CA LERICKSO at CROP.UOGUELPH.CA
Wed Nov 3 08:05:39 EST 1993


> >
> > > Dear netters,
> > > After reading a paper by Michael Witty about Thaumatin II reporter
> > > gene utilisation in plant genetic manipulations (which appeared in
> > > Methods of Enzymology 216: 441-447, 1992), one particular paragraph
> > > at the Concluding Remarks section (p. 447) made me curious.
> > > At the final paragraph, the author mentioned  a phenotypic change in
> > > plants overexpressing the Thaumatin II under the control of CaMV35S,
> > > and continued to state that this phenotypic change could be used to
> > > detect transgenic lines or whole plants.
> > > Could someone inform me what this phenotypic change is all about?
> > > Is it a color change or more on the morphology level?
> > >
> > > With my best regards,
> > > Dr. Sertac Onde
> > > Middle East Technical University, Biology Dept.
> > > Ankara, Turkey.
> > >
> >  I would also be interested in the answer to this question.
> >
> > Stephen A. Pluhar
> > SPLUHAR at CROP.UOGUELPH.CA           Dept. Crop Science  U. of Guelph
> > Phone: 519-824-4120 Ext. 4865      Guelph, Ontario, Canada. N1G 2W1
> >
>     Of course the best source for an explanation is Michael Witty,
> but I suspect he simply meant that the change is to one of sweetness
> (of the thaumatin sort), and by "overexpression" he meant simply
> expression. My apologies to you and Michael if I am wrong, but I
> don't know if he uses these networks. I, too, am interested in such
> "sweet" proteins; perhaps we could begin a discussion/exchange on the
> topic and engage Michael Witty in it.
>

> Larry R. Erickson,
> Department of Crop Science,
> University of Guelph,
> Guelph, Ontario,
> Canada  N1G 2W1
> Internet: LERICKSO at CROP.UOGUELPH.CA
> Fax: 519-763-8933  Phone: 519-824-4120 Ext. 3398
>Larry R. Erickson,
Department of Crop Science,
University of Guelph,
Guelph, Ontario,
Canada  N1G 2W1
Internet: LERICKSO at CROP.UOGUELPH.CA
Fax: 519-763-8933  Phone: 519-824-4120 Ext. 3398



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