tobacco rRNA sizes ?
Leonard N. Bloksberg
bloksber at pilot.msu.edu
Tue Oct 26 12:00:00 EST 1993
In Article <1993Oct26.093336.583 at gserv1.dl.ac.uk> "suter at de.d400.mpg.MPIZ-KOELN.VAX" says:
> dear humans,
> on a northern i see with my probe two signals, of 1200 and 4800 nucleotides.
> as always, i am a little scared this could be the result of cross-hybridization
> with a very abundant RNA, like ribosomal.
> What are the sizes of ribosomal RNAs in tobacco ???????
> tia, clemens
> Dr. Clemens Suter-Crazzolara, PhD
> Max-Planck-Institut fuer Zuechtungsforschung
> Abteilung Genetische Grundlagen der Zuechtungsforschung
> Carl-von-Linne Weg 10, D-50829 Koeln, Germany
> Tel.xx49.221.5062-221 Fax.-213 e-mail: suter at vax.mpiz-koeln.mpg.d400.de
Well, since RNA tends to run oddly, and I don't know wether your standards
are ssDNA, dsDNA, or RNA, I can't rightly say. But, you could answer the
question yourself (and more accurately than I ever could) by looking at
your Ethidium bromide stained RNA gel. Where do the rRNA bands run? Do
they line up exactly with your mistery bands? If this is a problem, you
could try several things:
. 1. Use poly-A RNA instead of total.
. 2. Make sure your mRNA is still in tact (pos. cntrl probe).
. If not, make better quality RNA.
. 3. Find a homologous (not heterologous) probe.
. Leonard N. Bloksberg
. Bloksber at pilot.msu.edu
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