PCR in plant genomic DNA
preissj at clvax1.msu.edu
Tue Sep 7 13:03:00 EST 1993
In Article <9309031921.AA25960 at net.bio.net> "CHAIVISU at UCONNVM.bitnet (Parin)" says:
> Does anyone has a protocol for PCR in plant genomic DNA ? We have some
> problems. Our PCR control worked but the PCR in plant genomic DNA didn't work.
> Thank you
I had some problems getting this started too. It seems the problem was too
much template (genomic tomato DNA). Try adjusting template concentration.
A friend had some problems with introns and larger than anticipated products.
To overcome this, use longer elongation times. About 1 minute/kbp is a
Dr. Leonard N. Bloksberg
PreissJ at clvax1.cl.msu.edu
More information about the Plantbio