T phage

ralston at ROCKVAX.ROCKEFELLER.EDU ralston at ROCKVAX.ROCKEFELLER.EDU
Fri Sep 17 12:24:15 EST 1993


Dear Plant Netters,
Has anyone else out there purchased the Nicotiana tabacum genomic
library from Clontech?  If so, did you also find it contaminated
with large plaques?  This was my experience.  I found 1-5 large
plaques per 45,000-50,000 lambda plaques.

I have heard that T phage grow to similar size and that T phage
contamination is a real nightmare.  

Are these definately T phage?  What clean-up measures should I
take?  Can I make filters from these plates?  Will T phage
contaminate hybridizing ovens, heating ovens, 37 degree incubators?

Thanks for any suggestions,
Diana Horvath
ralston at rockvax.rockefeller.edu



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