brambl at graz.cbs.umn.edu
Wed Sep 22 13:10:25 EST 1993
In article <1993Sep21.161628.302 at gserv1.dl.ac.uk> ajt at uk.ac.sari.rri (Tony
> rogersh at uk.ac.afrc wrote:
> : Subject: mitochondrial volume
> : I sent this query to Biosci::methods & reagents and got little
> : so I thought I would broaden my search for a reply....
> : The poser I have is:
> : How would one go about determining the mitochondrial volume in
> : tissue culture cells....unfortunately plant ones.
> : Is there a method(s) for doing this ?
> : Has any one done it ?
Measuring mitochondrial volume in vivo can be tricky, since mitochondria
may change shape, fuse, shrink, or swell, depending upon physiological
conditions. Nevertheless, if you can hold all these things constant, I
would suggest using potentiometric cyanine dyes for the mitochondria.
These work beautifully in plant cells. See Plant Physiol. (1987)
84:1385-1390. You could photograph the fluorescent mitochondria, enlarge
the prints suitably, and use area measurements to *estimate* mitochondrial
Hope this helps.
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