Electofusion of plant proto's via BIORAD Gene Pulser??
jm15 at cornell.edu
Sun Aug 21 15:33:58 EST 1994
Hello Fellow Researchers:
I want to fuse tobacco protoplasts using a Biorad Gene Pulser. Biorad's
technical service/info. refers to experiments done by researchers several
years ago using old prototypes/homemade units. Thus, I am hoping someone
is using Biorad's Gene Pulser and is willing to share their optimization
parameters for both maximum transient reporter gene expression and
recovery of stably-transformed calli. If you have electrofusion
experience using plant protoplast other than tobacco, I welcome any
helpful comments you may have. Thanks.
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