BY2 cell extracts

Ann Loraine loraine at NATURE.BERKELEY.EDU
Fri Dec 16 17:27:17 EST 1994

Hi!  I am looking for a  method to make protein
extracts from tobacco BY2 cells.  The activity I want to examine is a
located in the soluable, or cytoplasmic, fraction of plant cells.  Can
someone send me a reference that could help?

I've also heard of a method that utilizes a centrifugation step to
separate vacuole from other cell contents as a way of removing vacuolar
proteases.  Has anyone heard of such a thing?  And if so, could you give
me a lead on where I can find info on how to do this?

Thanks in advance!

Ann Loraine
loraine at

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