BY2 cell extracts
loraine at NATURE.BERKELEY.EDU
Fri Dec 16 17:27:17 EST 1994
Hi! I am looking for a method to make protein
extracts from tobacco BY2 cells. The activity I want to examine is a
located in the soluable, or cytoplasmic, fraction of plant cells. Can
someone send me a reference that could help?
I've also heard of a method that utilizes a centrifugation step to
separate vacuole from other cell contents as a way of removing vacuolar
proteases. Has anyone heard of such a thing? And if so, could you give
me a lead on where I can find info on how to do this?
Thanks in advance!
loraine at nature.berkeley.edu
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