Acetone is usually used to remove low molecular weight polar materials such as
pigments and phenolics that may be present in flower extracts.
If you 're not sure about its importance, omit it and see what happens.
Efraim Lewinsohn
----------------------------Original message----------------------------
I realize for the moment proteins extraction from flower organs grinded in
liquid N2 with a boiling SDS buffer (4% SDS, 5% mercaptoethanol, 5%
sucrose) followed by incubation in an acetone buffer for one hour a -20!C.
So, question is : Is the acetone step necessary and if it is, what is its
interest?
Thanks in advance
D.M.
