Hi. This is a second request for a wound-inducible plant gene
promoter. Several people wanted more information, therefore this second
request.
I am working with antifungal peptides. I wish to make a gene
construct for one or more of these peptides and clone it into American
chestnut, to determine if they could increase disease resistance. As part
of these gene constructs, I would like to include a wound-inducible
promoter so that the peptides would primarily be produced at the sites of
infection.
I have already been in contact with Dr. Milton Gordon
concerning his win3 promoter. This promoter might work, but from Dr.
Gordon's and Jay Hollick's studies, it might cause these peptides to
accumulate in the seeds. I would prefer a more specific wound-inducible
promoter, if available. Therefore, I would like to know if there might be other
promoters I could try and where I could obtain them. I appreciate any
help you can give. Thanks.
Dr. William Powell
College of Environmental Science & Forestry, SUNY
Syracuse, NY 13210-2788
