Casper Linnestad CASPERL at BIOMED.UIO.NO
Tue Jan 18 07:04:22 EST 1994

In <2hge8q$efv at> Egea writes:

> does anybody have a clue or suggestion of an EcoK r-m- strain to 
> use as a host for cloning?.  I have a ClaI methylated that need to 
> unmethylate to cut and subclone.
> Thanks 
> Marcos Egea Cortines
Hi there !
I think one solution to your problem is to use the E.Coli K12 strain
HB101 which is EcoK R-M-.
Good luck!

By the way, I think HB101 is easy to transform, I haven't tried it myself

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