EcoKR-M_

Bonham, Peta pbonham at pbi.nrc.ca
Tue Jan 18 11:47:56 EST 1994


Hello Marcos:
I had a similar problem and I took my plasmid containing the methylated ClaI 
site out of DH5alpha and put it into BW58, isolated plasmid DNA and now it 
cuts perfectly.
BW58 is dcm- and dam-
don't know the rest of its genotype
Peta Bonham-Smith
Biomira Inc.
pbonham at pbi.nrc.ca
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> does anybody have a clue or suggestion of an EcoK r-m- strain to 
> use as a host for cloning?.  I have a ClaI methylated that need to 
> unmethylate to cut and subclone.
> 
> Thanks 
> Marcos Egea Cortines
> Hi there !
> I think one solution to your problem is to use the E.Coli K12 strain
> HB101 which is EcoK R-M-.
> Good luck!
> Casper.

By the way, I think HB101 is easy to transform, I haven't tried it myself
C.
though.






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