Isolation of Mitochondria using Percoll gradients

Roy Navarre navarrer at ava.bcc.orst.edu
Thu Jan 27 19:54:41 EST 1994


We isolate mitochondria from green oat leaves using standard
techniques with 28% Percoll gradients to purify the mitochondria away
from the chloroplasts.  There is a hard white/grey pellet on the
bottom of these percoll gradients.  A tight pellet on the side of the
tube bottom which wont pour or wash out easily.  Does anyone who
does organelle isolations know what this pellet is?
We are interested in the mitochondrial band, but are curious about
the identity of the pellet.  Current guess is nuclei and/or
peroxisomes.

Roy
navarrer at ava.bcc.orst.edu
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