Leaf clearing techniques?
barosb at alleg.edu
Wed Jul 20 13:42:20 EST 1994
I'm doing research with epidermal and mesophyll cells, especially
counting cells per unit area after ABA treatment in Hippuris
vulgaris...does anybody have a good, quick clearing method that will
remove everything excpet cell walls? I've been using 90% EtOH followed
by 7% NaOH, but it doesn't clear enough that I can see mesophyll cell
walls. Any suggestions? Most of the readings that I've looked at are
for the staining of xylem, but I don't need to see the xylem, and some
even degrade cellulose, which for me would be bad.
Brandi Baros barosb at alleg.edu
Allegheny College Bio dept. undergrad
More information about the Plantbio