Leaf clearing techniques?
Biomancer
barosb at alleg.edu
Wed Jul 20 13:42:20 EST 1994
I'm doing research with epidermal and mesophyll cells, especially
counting cells per unit area after ABA treatment in Hippuris
vulgaris...does anybody have a good, quick clearing method that will
remove everything excpet cell walls? I've been using 90% EtOH followed
by 7% NaOH, but it doesn't clear enough that I can see mesophyll cell
walls. Any suggestions? Most of the readings that I've looked at are
for the staining of xylem, but I don't need to see the xylem, and some
even degrade cellulose, which for me would be bad.
--
Brandi Baros barosb at alleg.edu
Allegheny College Bio dept. undergrad
More information about the Plantbio
mailing list