Postdoctoral positions - Chloroplast biogenesis

David B. Stern ds28 at cornell.edu
Thu Jun 9 16:46:52 EST 1994


Postdoctoral Positions in Chloroplast Biogenesis

2-3 postdoctoral positions will be available beginning September 1, 1994,
to use molecular genetic approaches to study two aspects of chloroplast
biogenesis in the unicellular eukaryotic alga Chlamydomonas reinhardtii.  

In the first project, chloroplast transformation approaches will be used
to define chloroplast transcript features that regulate translation
initiation and mRNA stability (see PNAS 90:497; EMBO J. 12:3627).  
Nuclear transformation will be used to generate insertional mutations 
and to clone genes encoding trans-acting proteins that regulate 
these processes.  

In the second project, genetic approaches will be used to define
components of the chloroplast protein import pathway that 
interact with the transit peptides of cytoplasmically synthesized 
precursors. This project will involve 1) generating and testing transit 
peptide mutations in vivo and in vitro and 2) isolating extragenic 
suppressors that can compensate for the transit peptide
mutation.  

The positions will be supported for a period of up to three years.  
Experience in molecular biology and either protein biochemistry 
or genetics is required.  Qualified individuals should send a statement
of research interests, curriculum vita, and the names, addresses and
phone numbers of three references to:

Project 1: Dr. David Stern, Boyce Thompson Institute, Cornell University,
Tower Rd., Ithaca NY 14853; Phone: (607) 254-1306; e-mail: 
ds28 at cornell.edu; Fax (607) 254-1242); or 

Project 2: Dr. Karen Kindle, 151 Biotechnology Building, Cornell
University,
Ithaca, NY  14853; Phone (607) 254-4851; e-mail:
Karen_Kindle at qmrelay.mail.cornell.edu; Fax (607) 254-2428. 

"The Boyce Thompson Institute and Cornell University are affirmative
action and equal opportunity employers; women and minorities are
encouraged to apply.



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