Field sampling of maize tissue for DNA
Julian Paul Robinson
jpr at st-andrews.ac.uk
Wed Jun 15 04:50:09 EST 1994
On Tue, 7 Jun 1994, TINKER wrote:
> I would appreciate hearing from anyone with experience or ideas
> on sampling plant (especially maize) tissue in the field for
> future DNA extraction.
> Nick Tinker
> tinker at agradm.lan.mcgill.ca
Without wishing to blow my own trumpet to much I suggest you read
either of the following papers and the references contained within.
Harris and Robinson (1994),Preservation of Tropical Plant Material for
Molecular Analyses'. Proceedings of the 2nd DNA Bank-Net Meeting.
Harris (1993), 'DNA analysis of tropical plant species: An assesment of
different drying methods'. Plant Systematics and Evolution
Both Harris and I were(are) working on woody tropical legumes, we
needed DNA from taxa that we were unable to collect seed material from for
temporal reasons, or from sterile hybrids. This led to us investigating methods
of collecting leaf material in the field and preserving it until we could
transport it back to the lab to extract the DNA.
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