Sugar beet arginase extraction

P.Longdon cs6170 at
Tue Jun 28 09:28:11 EST 1994

Dear All,
      I have just started my work experience placement as a Research 
Assistant and will be looking at the effects of nitrogen, sulphur and
potassium on the free amino acid content of sugar beet.
  I would also like to do assays on arginase activity, and this is really
the bit for which I could do with some tips: how would I go about extracting
and/or purifying this enzyme (a crude extract is really all I'd need) from
the leaves of the plant?


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