suspension cultures

Jacqueline Bede bedejc at
Wed Jun 29 14:14:51 EST 1994

Hi !
I'm trying to extract sesquiterpenoids from plant suspension
cultures and had a few questions regarding different procedures.
1. Which procedure is most suitable for extraction
homogenization vs sonication vs grinding in liquid nitrogen or are
they all acceptable as long as the cell is disrupted ?
2. What staining techniques are acceptable for determination of c
cell number for growth curves ? 
3. How are solutions containing 2,4-D disposed of ?
Thanks in advance,

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