TLC of spinach pigments
eeyore at wpi.WPI.EDU
Sun Mar 6 12:35:24 EST 1994
I've been trying to separate and quantitate pigments from spinach
leaves (xanthophylls, chlorophyll b, chlorophyll a, pheophytin, carotenoids)
I am finding all but the chlorophylls separate. The pigments are extracted
into 100% acetone (25 g deveined leaves in 100 mL acetone) in a blender
or homogenizer. 10 mL of hexane are added, and this portion is separated
and retained. 30 uL of this extract is spotted onto a silica gel TLC plate
and the solvent system is 3:1:1 petroleum ether:chloroform:acetone.
Any idea on what the problem might be?
a TA trying to get this to
work before the students
have to try it!
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