Long seq/runs faint
marcos at techunix.technion.ac.il
Tue Nov 22 05:57:10 EST 1994
I have a problem with my sequencing gels. Even when the
reactions look perfect and strong, if we try to run them long, the whole
thing just faints, and we hardly can read the longest runs. The short
ones are OK. We have an Owl system with
43cm long gels, and run the standard 6% Acryl etc. Short runs will take
two hours at about 50V/cm (2300 V). The recommended speed in the Red
Book is 70 V/cm, but they get very hot at this voltage.
Does anybody know how to overcome this problem?
Thanks in advance
Dr. Marcos Egea Cortines
Israel Institute of Technology
Dept. of Biology 32000 Haifa
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