Ti plasmid isolation

malamy at mcclb0.med.nyu.edu malamy at mcclb0.med.nyu.edu
Mon Oct 10 09:22:59 EST 1994

We use Wizard DNA minipreps from Promega to isolate Agro plasmids.  Before
this we always had considerable contamination with chromosomal DNA, but
this method gives us excellent results.  It also gives a reasonably good
yield.  We follow the method exactly as described in the kit, although I
often start with more cells than they do.    Jocelyn

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