protoplast culture

Oscar Jaimes Suarez ojaimes at IVIC.IVIC.VE
Mon Sep 19 15:27:30 EST 1994

 Hi,I have been working with tomato protoplast during a few months and I noticed
that when cultivating them (after isolation) within a week a kind of budding
takes place.I use TM-2 medium for protoplast culture and W-5 medium for purifi-
cation.Can anyone help me with this problem or at least give some recomendations?

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