DNA extractions

bio_hannan at emuvax.emich.edu bio_hannan at emuvax.emich.edu
Thu Dec 7 20:39:08 EST 1995


In article <casper-0512951126180001 at 137.131.36.12>, casper at scripps.edu (Steven Casper) writes:
> In article <1995Dec4.213748 at emuvax.emich.edu>, bio_hannan at emuvax.emich.edu
> wrote:
> 
>> In article <49n9o2$7tv at mserv1.dl.ac.uk>, "Corinna. Dutton"
> <Corinna.Dutton at newcastle.ac.uk> writes:
>> > I have had problems purifying genomic DNA for southerns from potato.. 
> ...............
>> You might want to try BioClean ..........
> 
>    Hi, 
>  
>    I would like to add a caution to the BioClean purification method.  The
> glassmilk is actually small silicate shards with rough, sharp edges. 
> While this substance works very well in the purification of plasmid DNA, I
> would not necessarily use it for genomic.  Sharp micro objects and long
> DNA strands don't go well together, in my opinion.  If this is used in
> purification, extreme care must be applied when the glassmilk is in
> contact with the DNA to reduce shearing or nicking.
> 
>    Good Luck,
>    
>    Steven
That is possible.  So we have always used the same protocol througout our
preps that will be compared to each other.  Nothing else seemed to clean up
the DNA enough to allow amplification and to not produce streaking.

Gary Hannan



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