tomato transformation

Hwang Jongpil z942460 at AXP3400.KYUNGPOOK.AC.KR
Thu Feb 9 06:15:04 EST 1995


   Hello Everyone
 
   Could someone answer a  question on tomato transformation?
   Lycopersion Pennellii. It is easy to induce callus and shoot from explant
   I  succeed  in  making  some  plants  from  explant(leaflet)  of  Lycopersion                   
   Pennellii.
   They was selected in KM 50mg/L(kanamycin) during inducing shoot, callus 
   but GUS expression is negative although T-DNA have a NPTII and Gus gene.
   I don't experiment Southern Blot Analysis or PCR yet!!
   How percent(%) do I guess  about a transgenic tomato ?
   Have you ever gotten the same result?
   could someone explain it to me?
   I would appreciate any information on this subject.
   
   
   Hwang Jong Pil
   e-mail: z942460 at axp3400.kyungpook.ac.kr





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