Bizarre Transgene Idea?
Chris_Jones at nottingham.ac.uk
Thu Feb 16 14:25:55 EST 1995
In article <3htugf$aou at emory.mathcs.emory.edu> medtjm at bimcore.emory.edu (T. J. Murphy) writes:
>From: medtjm at bimcore.emory.edu (T. J. Murphy)
>Subject: Bizarre Transgene Idea?
>Date: 15 Feb 1995 22:16:47 GMT
>Problem: I need massive quantities of a mammalian integral membrane protein.
> Bacteria can't handle the protein.
>Question: Could I make a transgenic tomato to overexpress my protein as a
> source for purification? Has this been attempted? Any pro's
> or con's?
If yeast don't work then think about baculoviruses but I am not sure how they
tolerate membrane proteins. I guess you know that membrane proteins are amongt
the most difficult to express....
Oh, Yes. Just how much is massive? and whats the coli expression problem. You
could try a fusion in coli, fusions often lack the lethality that some prots
show. Good luck
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