Bizarre Transgene Idea?
patrick at corona
Wed Feb 15 21:29:29 EST 1995
On 15 Feb 1995, T. J. Murphy wrote:
> Problem: I need massive quantities of a mammalian integral membrane protein.
> Bacteria can't handle the protein.
> Question: Could I make a transgenic tomato to overexpress my protein as a
> source for purification? Has this been attempted? Any pro's
> or con's?
I am not really well acquainted with plants, at least as far as for the
purpose of an expression system, but I could suggest, assuming you haven't
considered it yet for some reason, the yeast Saccharomyces cerevisiae before
going on to the more complex and difficult.
It's certainly quicker than a tomato, if there is nothing to prevent its
expression or folding.
More information about the Plantbio