Bizarre Transgene Idea?

Raymond Shillito TMBG42A at prodigy.com
Fri Feb 24 00:11:47 EST 1995


I would think the best approach in any system is to express it from an 
inducible promoter with a very low constitutive level.  This is easiest 
in bacteria, but you can od it in yeast etc. too.  The same would apply 
in plants. If it messes up membranes, then you have to go the inducible 
route.

As for choice of plant - go with leaf expression in tobacco, you would 
get masses of easily extracted tissue.  Maybe you could export it from 
the cells into the intracellular space, or the culture medium in the case 
of yeast, bacteria or animal cells.


RAYMOND SHILLITO  shillitor at am.abru.cg.com





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