isolation of nucleus of seed cells

consuelo medeiros rodrigues de lima corn-017 at GUARANY.CPD.UNB.BR
Wed Jul 26 17:12:35 EST 1995

dear netters
I am working with a leguminous seed protein that seems to occur at 
the nucleus and cytosol. In order to confirm the nuclear localization
I need a very pure nuclear fraction. So I would like very much if 
someone could send me a protocol or references concerning nucleus
purification of seed cells.  

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