isolation of nucleus of seed cells (fwd)

consuelo medeiros rodrigues de lima corn-017 at GUARANY.CPD.UNB.BR
Thu Jul 27 13:34:10 EST 1995

Forwarded message:
> From BIOSCI-REQUEST at Wed Jul 26 19:50:13 1995
> To: plant-biology at
> From: corn-017 at (consuelo medeiros rodrigues de lima)
> Subject: isolation of nucleus of seed cells
> Date: 26 Jul 1995 15:12:35 -0700
> Sender: daemon at
> Message-Id: <9507262106.AA60913 at>
> Nntp-Posting-Host:
> dear netters
> I am working with a leguminous seed protein that seems to occur at 
> the nucleus and cytosol. In order to confirm the nuclear localization
> I need a very pure nuclear fraction. So I would like very much if 
> someone could send me a protocol or references concerning nucleus
> purification of seed cells.  
>                             Thanks, 

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