isolation of nucleus of seed cells (fwd)

[consuelo medeiros rodrigues de lima]

Forwarded message:
> From BIOSCI-REQUEST at net.bio.net Wed Jul 26 19:50:13 1995
> To: plant-biology at net.bio.net
> From: corn-017 at guarany.cpd.unb.br (consuelo medeiros rodrigues de lima)
> Subject: isolation of nucleus of seed cells
> Date: 26 Jul 1995 15:12:35 -0700
> Sender: daemon at net.bio.net
> Message-Id: <9507262106.AA60913 at guarany.cpd.unb.br>
> Nntp-Posting-Host: net.bio.net
> 
> dear netters
> I am working with a leguminous seed protein that seems to occur at 
> the nucleus and cytosol. In order to confirm the nuclear localization
> I need a very pure nuclear fraction. So I would like very much if 
> someone could send me a protocol or references concerning nucleus
> purification of seed cells.  
> 
>                             Thanks, 
                                       Consuelo.