Toxicity Testing in Plants
Mary Elyse Lucero
malucero at nmsu.edu
Wed Jul 26 22:24:16 EST 1995
Thanks for the tips. I have also been using FDA staining, and
yes, it does do a good job as far as identifying viable vs. inviable
cells. I have also tried bromophenol blue staining, but FDA gives more
Your observation about round cells is interesting. In general, my
cells (Datura innoxia) are round, but I may see more round cells after
treatment too, I'll have to pay more attention.
Do you notice a tendency for cells in suspension to aggregate more
when treated with toxins? How about changes in cell size? Mine tend to
swell up at sub-lethal doses.
Molecular Biology & Environmental Toxicology
More information about the Plantbio