Help with leaf disk assay?
James Thomas Murphy
jtmurphy at CATS.UCSC.EDU
Tue Jun 20 12:55:19 EST 1995
Hello plant people,
I am supposed to come up with a leaf disk assay in which a leaf disk
from a raspberry seedling is innoculated with a rust fungi. The problem
is that the leaf disk can not dessicate or otherwise deteriorate and the
assay has to last about 10-14 days. I can't use whole seedlings for the
assay, I have to use leaf disks. I've done a literature search but the
references I've found aren't at my school. Can anyone point out some
references or give advice on coming up with a good technique for
maintaing a leaf disk in "good" condition for up to two weeks? Any help
would be greatly appreiated.
jtmurphy at cats.ucsc.edu
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