wylie at CENTRAL.MURDOCH.EDU.AU
Tue Oct 10 02:31:28 EST 1995
Does anyone else working with Agrobacterium tumefaciens have the
problems that I do?
Firstly it is very inconsistant in growth, sometimes
growing vigorously in liquid medium and at other times growing slowly.
Transforming it with large plasmids with electroporation or triparental
mating has been impossible with some plasmids but very easy with others
of comparable size. All conditions and cells identical.
I use a strain call Agli and have not tried others. I guess I should try
I store a glycerol stock at -80 C and plate onto LB + glucose every
couple of weeks, working from single colonies taken from that plate.
Any suggestions most welcome. Thanks.
More information about the Plantbio