Fluorescent GUS?

Francisco Jose Lima Aragao aragao at cenargen.embrapa.br
Mon Apr 1 08:53:05 EST 1996


Yes, beta-gluc. form a flourecente product called MU, que substrate is MUG.
But I have no idea if the assay coul be non-destructive. 
Francisco.

*******************************************************
Francisco J. L. Aragao
EMBRAPA-CENARGEN-ABC
 SAIN Parque Rural C.P. 02372
 70849-970 Brasilia, DF, Brazil.
 FAX : +55-61-3403624 (new)!!!!!!!!!!!!!
*******************************************************

On Sun, 31 Mar 1996, TJ Murphy wrote:

> Can beta-glucuronidase form a fluorescent product and in a manner that 
> won't kill cells?  I would like to express GUS in mammalian cells, then 
> isolate transgenic cells by FACS.
> 



More information about the Plantbio mailing list