tissue culture!

arrie at scientia.up.ac.za arrie at scientia.up.ac.za
Fri Aug 16 08:51:44 EST 1996


>I am trying my damdest to get sterile mature explants of Nepenthes alata, 
>but the material is being killed by the sterilant, while the contaminants 
>are still alive and well, any ideas?  I've tried mercuric chloride, sodium 
>hypochlorite (3.5%), hydrogen peroxide, benlate and other dydtemic 
>fungicides..

>Any help would be appriciated.

>Lindsey Podd
>Department of Botany,
>Pietermaritzburg campus,
>University of natal
>South Africa


I don't know the plant you working with but what about seeds as explants?
Seeds can often be sterilized with higher concentrations than the plant it 
self can.

Goodluck


Arrie
Genetics
UP
RSA



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