Histochemical staining advise
Gary V. Deutschmann, Sr.
raiar at inlink.com
Mon Feb 19 21:14:25 EST 1996
L.Bloksberg at genesis.co.nz (Dr Leonard N. Bloksberg) wrote:
>First, thanks to those who recomended D. A. Johansen's book
>"Plant Microtechnique" (McGraw Hill, NY 1940) as a manual
>for histochemical staining plant material to differentiate
>phloem and xylem. I have found many protocols which appear
>to meet my needs.
> Now I have some questions. Many of the protocols call
>Picric acid (highly explosive) and chromic acid (delequescent
>and carcinogenic). I may not even be able to get these chemicals
>and would like to avoid them anyway.
> The picric acid is usually used as picric acid saturated
>alcohol to resolve safranin stained tissue. Can I substitute
>a little nitric acid, or nitric acid and phenol instead?
> The chromic acid is used to macerate tissue as a solution
>of 10% nitric acid and 10% chromic acid. Can I substitute
>10% sulfuric for the chromic to macerate woody tissue to view
> Please send responses directly to my email address. Thanks
>L.Bloksberg at genesis.co.nz
I would say try it and see!
I wouldn't worry too much about picric acid and the flamability
thereof, I used it daily for over 7 years with a cigarette hanging out
of my mouth and a bunsen stem sealer plate right next it and I'm still
Anything made with water is carcinogenic so I wouldn't worry about
that too much either.
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