vital stain for protoplasts
Charles S Buer
buer at WPI.EDU
Wed Jan 31 16:04:24 EST 1996
Several dyes work on the exclusionary basis. Trypan blue, Evan's blue,
and phenosafranine should all work. I have only used trypan blue. I
plan to test the other two, but haven't received them as yet.
Fluorescein diacetate could also be used, but a UV light source is
necessary to observe the fluorescence of viable cells.
REF: Widholm, J.M. 1972. Stain Technology. 189-194. They are all
available from Sigma. I don't know about neutral red.
Worcester Polytechnic Institute
On Wed, 31 Jan 1996, Tony Travis wrote:
> Alyson Tobin wrote:
> > We are working with isolated barley leaf protoplasts and need to stain them
> > to test for intactness of the plasmamembrane. I'm sure there is a suitable
> > dye but can't remember the name of it . Can anyone help with a name,
> > supplier and/or reference for the method please?
> > Alyson Tobin (St Andrews University)
> I think the stain you want is neutral red (available from Sigma).
> Dr. A.J.Travis, | mailto:ajt at rri.sari.ac.uk
> Rowett Research Institute, | http://www.rri.sari.ac.uk/~ajt
> Greenburn Road, Bucksburn, | phone:+44 (0)1224 712751
> Aberdeen AB2 9SB, Scotland, UK. | fax:+44 (0)1224 716687
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